Correction form 4
PKBS2 Biology
Part D (Experiment)
1. Objective | To study the effect of albumen concentration on the rate of pepsin Reaction. | |||||||||||||||
| 2. Problem Statement | What is the effect of albumen concentration on the rate of pepsin reaction? | |||||||||||||||
| 3. Hypothesis | As the concentration of albumen increases, the rate of pepsin reaction will increase ( until the reaction reaches a maximum rate) | |||||||||||||||
| 4. Variables | Manipulated variable : Concentration of albumen Responding variable : Time taken for the albumen to turn clear // Rate of pepsin reaction . Fixed variable : Enzyme concentration/ temperature / pH of medium (pH 2) | |||||||||||||||
| 5. Materials and apparatus | Albumen suspension: 5%, 10% , 15% and 20% , Pepsin (1.0 % ) , Hydrochloride acid (HCl) , distilled water, syringes, test tubes, stopwatch, water bath and thermometer. | |||||||||||||||
| 6. Technique | Measure and record the time taken for the albumen suspension to turn clear using a stopwatch.// Calculate the pepsin reaction by using a formula : 1/ time ( min -1) | |||||||||||||||
| 7. Procedure | 1. Four test tubes labeled A to D are prepare. 2. The test tubes contain the following mixtures :- A : 4 ml of 5 % albumen suspension B : 4 ml of 10 % albumen suspension C : 4 ml of 15 % albumen suspension D : 4 ml of 20 % albumen suspension 3. I ml of 1% pepsin is added to each test tube by using a syringe.
1. Add 3 drops of HCl to all test tubes to make the acidic medium 2. All the test tubes are immersed in a water bath set at 37ºC 3. The stopwatch is started and the time taken for the mixture to turn from cloudy (albumin / protein) to clear in each test tube is taken and recorded in table. | |||||||||||||||
| 8. Results |
| |||||||||||||||
| Discussion | …No discussion because you did not do the experiment… | |||||||||||||||
| 9. Conclusion | As the concentration of albumen increases, the rate of pepsin reaction will increase until the reaction reaches a maximum rate. Hypothesis is accepted. |
